Deoxyribonucleic acid-dependent nucleotide phosphohydrolase activity in purified vaccinia virus.
نویسندگان
چکیده
A nucleotide phosphohydrolase (adenosine triphosphatase), which is associated with vaccinia virus cores, has been solubilized and shown to be deoxyribonucleic acid dependent.
منابع مشابه
Regulation of synthesis of two immunologically distinct nucleic acid-dependent nucleoside triphosphate phosphohydrolases in vaccinia virus-infected HeLa cells.
The two nucleic acid-dependent nucleoside triphosphate phosphohydrolases, previously purified from vaccinia virus cores, were shown to be immunologically distinct enzymes. Antiserum prepared against purified phosphohydrolase I and antiserum prepared against purified phosphohydrolase II only neutralized the activity of that enzyme used as antigen. Both enzymes were induced in HeLa cells after va...
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The two purified nucleoside triphosphate phosphohydrolases from vaccinia virus are distinct enzymes as judged by their nucleotide substrate and polynucleotide cofactor specificities. Phosphohydrolase I hydrolyzes only ATP and dATP, whereas phosphohydrolase II hydrolyzes the four common ribonucleoside triphosphates as well as dATP and TTP. The range of K, values for ATP and dATP hydrolysis by ph...
متن کاملSeparation of a new deoxyribonucleic acid polymerase from vaccinia-infected HeLa cells.
HeLa cell deoxyribonucleic acid (DNA) polymerase was purified about 100-fold by sequential column chromatography on phosphocellulose, hydroxylapatite, and Bio Rex 70. A new form of DNA polymerase found in vaccinia virus-infected cells was separated from HeLa DNA polymerase by chromatography on diethylamino-ethyl cellulose. The new form was also purified approximately 100-fold in the same manner...
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The presence of adenosine triphosphate, guanosine triphosphate, cytosine triphosphate, or uridine triphosphate reduced the rate of inactivation of vaccinia when heated at 50 C. The virus-associated nucleoside triphosphate phosphohydrolases (adenosine triphosphatase, guanosine triphosphatase, cytosine triphosphatase, and uridine triphosphatase) and ribonucleic acid polymerase were also protected...
متن کاملMutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase.
Vaccinia virus nucleoside triphosphate phosphohydrolase II (NPH-II), a 3'-to-5' RNA helicase, displays sequence similarity to members of the DExH family of nucleic acid-dependent nucleoside triphosphatases (NTPases). The contributions of the conserved GxGKT and DExH motifs to enzyme activity were assessed by alanine scanning mutagenesis. Histidine-tagged versions of NPH-II were expressed in vac...
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عنوان ژورنال:
- Journal of virology
دوره 10 4 شماره
صفحات -
تاریخ انتشار 1972